电针对糖尿病周围神经病变大鼠脊髓脂氧合酶的影响

文章编号：1005-5304（2018）05-0056-05

Effects of Electroacupuncture Therapy on Expression of Spinal Lipoxygenase

in Rats of Diabetic Peripheral Neuropathy

WANG Zhi-fu1， YANG Yi-zhou1， LIU Jian-bo1， LI Chang-zheng1， GONG De-gui2， YU Xiang-mei1

1. Integrative Medicine College， Fujian University of Traditional Chinese Medicine， Fuzhou 350122， China；

2. University of Traditional Chinese Medicine Subsidiary Rehabilitation Hospital， Fuzhou 350003， China

Abstract： Objective To observe the effects of electroacupuncture therapy on the expression of spinal lipoxygenase （LOX） in rats of diabetic peripheral neuropathy （DPN）； To discuss its mechanism in intervening DPN. Methods Thirty experimental rats were randomly divided into five groups： normal， DPN， electroacupuncture， LXA4 and Baicalein， 6 rats in each group. The model group was injected intraperitoneally with streptozotocin （STZ）， and the animal model of DPN was screened by blood sampling of tail vein with blood glucose， without intervention. Electroacupuncture was applied to bilateral “Shenshu” and “Zusanli” acupoints in electroacupuncture group for two weeks. The LXA4 and Baicalein groups were successfully intrathecal injected 3 times with LXA4 and Baicalein in the DPN rats. The paw withdrawal threshold was measured with electronic Frye fibers. The mRNA levels of LOX in the L4～5 spinal cord of DPN rats were detected with real-time PCR analysis. The protein concentration of sciatic nerve MBP was detected with ELISA analysis. Results Compared with the normal group， the mechanical pain threshold and sciatic nerve MBP content in the model group significantly decreased （P<0.01）， and the levels of 5-LOX， 12-LOX and 15-LOX mRNA in the spinal cord of the model group significantly increased （P<0.01）. Compared with model group， the mechanical pain threshold and MBP content of sciatic nerve in electroacupuncture group， LXA4 group and Baicalein group significantly increased， and the 5-LOX mRNA level in spinal cord of LXA4 group significantly